![]() The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives. UDG included for carryover contamination control Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality (see figure). In addition, this master mix provides efficient amplification over 6 logs of sample input. PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested (see figure). Tight reproducibility in CTs over a wide dynamic range In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification. PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding. Compatibility with most real-time qPCR instrumentsĮxceptional specificity with dual hot-start mechanism.Stability of pre-assembled reactions for up to 72 hours.Inclusion of UDG to help prevent carryover contamination.Highly reproducible CTs over a broad dynamic range.A dual hot-start mechanism for excellent specificity. ![]() ![]() PowerUp™ SYBR™ Green Master Mix features include: Coupled with user-supplied primer sets and template, PowerUp™ SYBR™ Green Master Mix is designed to amplifiy targets for accurate gene expression analysis. PowerUp™ SYBR™ Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
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